Review



two photon laser scanning microscope system  (Bruker Corporation)


Bioz Verified Symbol Bruker Corporation is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Bruker Corporation two photon laser scanning microscope system
    Two Photon Laser Scanning Microscope System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/Ultima+Investigator/bio_rxiv__64898__2026__03__29__715075-99-11-16
    Average 96 stars, based on 161 article reviews
    two photon laser scanning microscope system - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Laser-Scanning Microscopy:

    Article Title: Gas7 Is a Novel Dendritic Spine Initiation Factor
    Article Snippet: .. At 10–13 DIV, neurons were imaged with a two-photon laser-scanning microscope (Bruker Ultima Investigator) using a 60×, 0.9 numerical aperture objective (Olympus) using a Ti:Sapphire laser (Mai Tai Deep See Laser, Newport-Spectra Physics) at 920 nm with a imaging intensity of 20mW, measured in objective back aperture. .. Green and red fluorescence were captured with dual close-proximity photomultiplier GaAsP detectors (Hamamatsu Model H10770) using emission filters (et525/70m-2p and et595/50m-2p) and a t565lpxr dichroic beam splitter for simultaneous viewing and acquisition from both detectors.

    Article Title: Mushroom body input connections form independently of sensory activity in Drosophila melanogaster
    Article Snippet: Immobilized flies were exposed to two odors — isopentyl acetate (Sigma-Aldrich, 112674) diluted 5% volume to volume in paraffin oil (Fluka Analytical, 76235) and acetic acid (Fisher Scientific, A385) diluted in 5% volume to volume in water — using a stimulus controller (Ockenfels Syntech, CS 55). .. For imaging calcium transients, we used a two-photon laser scanning microscope (Bruker, Ultima Investigator) equipped with an ultrafast Chameleon Ti:Saphirre laser (Coherent) modulated by Pockels Cells (Conotopics). ..

    Article Title: Gas7 Is a Novel Dendritic Spine Initiation Factor
    Article Snippet: .. At 10–13 DIV, neurons were imaged with a two-photon laser-scanning microscope (Bruker Ultima Investigator) using a 60 , 0.9 numerical aperture objective (Olympus) using a Ti:Sapphire laser (Mai Tai Deep See Laser, NewportSpectra Physics) at 920nm with a imaging intensity of 20mW, measured in objective back aperture. .. Green and red fluorescence were captured with dual close-proximity photomultiplier GaAsP detectors (Hamamatsu Model H10770) using emission filters (et525/70m-2p and et595/50m-2p) and a t565lpxr dichroic beam splitter for simultaneous viewing and acquisition from both detectors.

    Article Title: Social state gates vision using three circuit mechanisms in Drosophila
    Article Snippet: .. Male imaging experiments were performed with an Ultima Investigator or Ultima Investigator Plus two-photon laser scanning microscope (Bruker Nanosystems) with a Chameleon Ultra II Ti:Sapphire laser. .. All samples were excited at a wavelength of 920 nm, and emitted fluorescence was detected with a GaAsP photodiode detector (Hamamatsu).

    Article Title: Multimodal cell type correspondence by intersectional mFISH in intact tissues
    Article Snippet: .. Virally transduced cells were visualized through a 40× 1.0 NA water immersion objective (Zeiss) on a two photon laser scanning microscope (Bruker Corp) with a tunable pulsed Ti:Sapphire laser (Chameleon Ultra, Coherent). .. Desired cells were individually photostimulated with a 1060 nm pulsed laser (Fidelity Femtosecond, Coherent) using galvo mirrors controlled by PrairieView software (Bruker Corp) to steer the beam through five revolutions of a 10 μm in diameter spiral over a 10 ms duration.

    Article Title: Male-male interactions shape mate selection in Drosophila
    Article Snippet: .. Functional imaging experiments were performed with an Ultima Investigator or Ultima Investigator Plus two-photon laser scanning microscope (Bruker Nanosystems) with a Chameleon Ultra II Ti:Sapphire laser. .. All samples were excited at a wavelength of 920nm, and emitted fluorescence was detected with a GaAsP photodiode detector (Hamamatsu).

    Imaging:

    Article Title: Gas7 Is a Novel Dendritic Spine Initiation Factor
    Article Snippet: .. At 10–13 DIV, neurons were imaged with a two-photon laser-scanning microscope (Bruker Ultima Investigator) using a 60×, 0.9 numerical aperture objective (Olympus) using a Ti:Sapphire laser (Mai Tai Deep See Laser, Newport-Spectra Physics) at 920 nm with a imaging intensity of 20mW, measured in objective back aperture. .. Green and red fluorescence were captured with dual close-proximity photomultiplier GaAsP detectors (Hamamatsu Model H10770) using emission filters (et525/70m-2p and et595/50m-2p) and a t565lpxr dichroic beam splitter for simultaneous viewing and acquisition from both detectors.

    Article Title: Mushroom body input connections form independently of sensory activity in Drosophila melanogaster
    Article Snippet: Immobilized flies were exposed to two odors — isopentyl acetate (Sigma-Aldrich, 112674) diluted 5% volume to volume in paraffin oil (Fluka Analytical, 76235) and acetic acid (Fisher Scientific, A385) diluted in 5% volume to volume in water — using a stimulus controller (Ockenfels Syntech, CS 55). .. For imaging calcium transients, we used a two-photon laser scanning microscope (Bruker, Ultima Investigator) equipped with an ultrafast Chameleon Ti:Saphirre laser (Coherent) modulated by Pockels Cells (Conotopics). ..

    Article Title: Gas7 Is a Novel Dendritic Spine Initiation Factor
    Article Snippet: .. At 10–13 DIV, neurons were imaged with a two-photon laser-scanning microscope (Bruker Ultima Investigator) using a 60 , 0.9 numerical aperture objective (Olympus) using a Ti:Sapphire laser (Mai Tai Deep See Laser, NewportSpectra Physics) at 920nm with a imaging intensity of 20mW, measured in objective back aperture. .. Green and red fluorescence were captured with dual close-proximity photomultiplier GaAsP detectors (Hamamatsu Model H10770) using emission filters (et525/70m-2p and et595/50m-2p) and a t565lpxr dichroic beam splitter for simultaneous viewing and acquisition from both detectors.

    Article Title: Social state gates vision using three circuit mechanisms in Drosophila
    Article Snippet: .. Male imaging experiments were performed with an Ultima Investigator or Ultima Investigator Plus two-photon laser scanning microscope (Bruker Nanosystems) with a Chameleon Ultra II Ti:Sapphire laser. .. All samples were excited at a wavelength of 920 nm, and emitted fluorescence was detected with a GaAsP photodiode detector (Hamamatsu).

    Article Title: Male-male interactions shape mate selection in Drosophila
    Article Snippet: .. Functional imaging experiments were performed with an Ultima Investigator or Ultima Investigator Plus two-photon laser scanning microscope (Bruker Nanosystems) with a Chameleon Ultra II Ti:Sapphire laser. .. All samples were excited at a wavelength of 920nm, and emitted fluorescence was detected with a GaAsP photodiode detector (Hamamatsu).

    Functional Assay:

    Article Title: Male-male interactions shape mate selection in Drosophila
    Article Snippet: .. Functional imaging experiments were performed with an Ultima Investigator or Ultima Investigator Plus two-photon laser scanning microscope (Bruker Nanosystems) with a Chameleon Ultra II Ti:Sapphire laser. .. All samples were excited at a wavelength of 920nm, and emitted fluorescence was detected with a GaAsP photodiode detector (Hamamatsu).



    Similar Products

    86
    Mayrhofer Pharmazeutika two photon laser scanning microscope
    Two Photon Laser Scanning Microscope, supplied by Mayrhofer Pharmazeutika, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/laser+microscopemayrhofer+photon+scanning+two/bio_rxiv__64898__2026__04__26__720930-168-7-10
    Average 86 stars, based on 1 article reviews
    two photon laser scanning microscope - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Bruker Corporation two photon laser scanning microscope system
    Two Photon Laser Scanning Microscope System, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/Ultima+Investigator/bio_rxiv__64898__2026__03__29__715075-99-11-16
    Average 96 stars, based on 1 article reviews
    two photon laser scanning microscope system - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Olympus two photon microscope
    Two Photon Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/FVMPE-RS+Multiphoton+Laser+Scanning+Microscope/pmc12296469-105-12-19
    Average 96 stars, based on 1 article reviews
    two photon microscope - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    86
    Scientifica two photon laser scanning microscope
    Two Photon Laser Scanning Microscope, supplied by Scientifica, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/microscope+photon+two/10__1016_slash_j__neuron__2026__02__038-339-12-15
    Average 86 stars, based on 1 article reviews
    two photon laser scanning microscope - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    96
    Olympus two photon laser scanning microscope
    Two Photon Laser Scanning Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/FVMPE-RS+Multiphoton+Laser+Scanning+Microscope/pm41667487-332-11-16
    Average 96 stars, based on 1 article reviews
    two photon laser scanning microscope - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    99
    Danaher Inc two photon laser scanning confocal microscope
    Two Photon Laser Scanning Confocal Microscope, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/DMi8+S+Inverted+Microscope+Solution/bio_rxiv__64898__2025__12__29__696858-319-6-10
    Average 99 stars, based on 1 article reviews
    two photon laser scanning confocal microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    99
    Nikon photon laser scanning confocal microscope
    Photon Laser Scanning Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/Objectives/bio_rxiv__64898__2025__12__24__696453-102-5-4
    Average 99 stars, based on 1 article reviews
    photon laser scanning confocal microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    Bruker Corporation two photon laser scanning microscope systems
    A. Angiogram generated with OCT imaging two months post-transplantation shown as (top) 500-µm maximum intensity projection along the Z axis (MIP Z ) and (bottom) as 100-µm maximum intensity projection along the X axis (MIP X ). The yellow dashed line indicates xenograft borders with the host cortex. The blue dashed line indicates the image segment corresponding to the bottom MIP X image. Schematic created in BioRender . Abbreviations: m , medial, c , caudal. B. Angiogram generated <t>with</t> <t>two-photon</t> (2P) imaging after intravenous injection of fluorescent Alexa-680 Dextran two months post-transplantation for the same animal as shown in panel A. The yellow dashed line indicates xenograft borders with the host cortex. C. Magnified 500-µm MIP Z angiograms showing capillaries within the xenograft as captured by OCT (top) and 2P microscopy (bottom) demonstrating strong agreement between the two modalities. D. Quantification of capillary density within the xenograft using 1 mm x 1 mm x 500 µm MIP Z OCT images at 1, 2, and 3 months after xenotransplantation. The plot shows the mean ± s.e.m. for three animals. E. Volumetric analysis of xenograft size 1, 2, and 3 months after xenotransplantation. (top) Logarithm-normalized, raw intensity, 100-µm MIP Z OCT images. The yellow dashed line indicates xenograft borders with the host cortex. (bottom) Manually segmented, 3D representations of the xenograft and the corresponding estimate of the xenograft volume.
    Two Photon Laser Scanning Microscope Systems, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/Ultima+Investigator/bio_rxiv__64898__2025__12__20__695690-204-10-15
    Average 96 stars, based on 1 article reviews
    two photon laser scanning microscope systems - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    99
    Olympus two photon confocal laser scanning microscope
    A. Angiogram generated with OCT imaging two months post-transplantation shown as (top) 500-µm maximum intensity projection along the Z axis (MIP Z ) and (bottom) as 100-µm maximum intensity projection along the X axis (MIP X ). The yellow dashed line indicates xenograft borders with the host cortex. The blue dashed line indicates the image segment corresponding to the bottom MIP X image. Schematic created in BioRender . Abbreviations: m , medial, c , caudal. B. Angiogram generated <t>with</t> <t>two-photon</t> (2P) imaging after intravenous injection of fluorescent Alexa-680 Dextran two months post-transplantation for the same animal as shown in panel A. The yellow dashed line indicates xenograft borders with the host cortex. C. Magnified 500-µm MIP Z angiograms showing capillaries within the xenograft as captured by OCT (top) and 2P microscopy (bottom) demonstrating strong agreement between the two modalities. D. Quantification of capillary density within the xenograft using 1 mm x 1 mm x 500 µm MIP Z OCT images at 1, 2, and 3 months after xenotransplantation. The plot shows the mean ± s.e.m. for three animals. E. Volumetric analysis of xenograft size 1, 2, and 3 months after xenotransplantation. (top) Logarithm-normalized, raw intensity, 100-µm MIP Z OCT images. The yellow dashed line indicates xenograft borders with the host cortex. (bottom) Manually segmented, 3D representations of the xenograft and the corresponding estimate of the xenograft volume.
    Two Photon Confocal Laser Scanning Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two+photon+laser+scanning+microscope/FV3000+Confocal+Laser+Scanning+Microscope/pm41365424-118-6-12
    Average 99 stars, based on 1 article reviews
    two photon confocal laser scanning microscope - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    A. Angiogram generated with OCT imaging two months post-transplantation shown as (top) 500-µm maximum intensity projection along the Z axis (MIP Z ) and (bottom) as 100-µm maximum intensity projection along the X axis (MIP X ). The yellow dashed line indicates xenograft borders with the host cortex. The blue dashed line indicates the image segment corresponding to the bottom MIP X image. Schematic created in BioRender . Abbreviations: m , medial, c , caudal. B. Angiogram generated with two-photon (2P) imaging after intravenous injection of fluorescent Alexa-680 Dextran two months post-transplantation for the same animal as shown in panel A. The yellow dashed line indicates xenograft borders with the host cortex. C. Magnified 500-µm MIP Z angiograms showing capillaries within the xenograft as captured by OCT (top) and 2P microscopy (bottom) demonstrating strong agreement between the two modalities. D. Quantification of capillary density within the xenograft using 1 mm x 1 mm x 500 µm MIP Z OCT images at 1, 2, and 3 months after xenotransplantation. The plot shows the mean ± s.e.m. for three animals. E. Volumetric analysis of xenograft size 1, 2, and 3 months after xenotransplantation. (top) Logarithm-normalized, raw intensity, 100-µm MIP Z OCT images. The yellow dashed line indicates xenograft borders with the host cortex. (bottom) Manually segmented, 3D representations of the xenograft and the corresponding estimate of the xenograft volume.

    Journal: bioRxiv

    Article Title: A neurorecording toolkit for longitudinal assessments of transplanted human cortical organoids in vivo

    doi: 10.64898/2025.12.20.695690

    Figure Lengend Snippet: A. Angiogram generated with OCT imaging two months post-transplantation shown as (top) 500-µm maximum intensity projection along the Z axis (MIP Z ) and (bottom) as 100-µm maximum intensity projection along the X axis (MIP X ). The yellow dashed line indicates xenograft borders with the host cortex. The blue dashed line indicates the image segment corresponding to the bottom MIP X image. Schematic created in BioRender . Abbreviations: m , medial, c , caudal. B. Angiogram generated with two-photon (2P) imaging after intravenous injection of fluorescent Alexa-680 Dextran two months post-transplantation for the same animal as shown in panel A. The yellow dashed line indicates xenograft borders with the host cortex. C. Magnified 500-µm MIP Z angiograms showing capillaries within the xenograft as captured by OCT (top) and 2P microscopy (bottom) demonstrating strong agreement between the two modalities. D. Quantification of capillary density within the xenograft using 1 mm x 1 mm x 500 µm MIP Z OCT images at 1, 2, and 3 months after xenotransplantation. The plot shows the mean ± s.e.m. for three animals. E. Volumetric analysis of xenograft size 1, 2, and 3 months after xenotransplantation. (top) Logarithm-normalized, raw intensity, 100-µm MIP Z OCT images. The yellow dashed line indicates xenograft borders with the host cortex. (bottom) Manually segmented, 3D representations of the xenograft and the corresponding estimate of the xenograft volume.

    Article Snippet: Two-photon imaging was performed in awake, head-fixed animals on commercial two-photon laser scanning microscope systems (Bruker Ultima, Bruker Ultima Investigator Plus) with Coherent Chameleon Ultra II or Coherent Chameleon Discovery Ti:Sapphire lasers tuned to 920-950 nm for excitation of GCaMP6s or 8s.

    Techniques: Generated, Imaging, Transplantation Assay, Injection, Microscopy

    A. Two-photon (2P) images from xenografts labelled by either AAV-hSyn-GCaMP8s (top) or LV-hSyn-GCaMP8s (bottom) in live mice one and three months after xenotransplantation. Images were generated from standard deviations of 3-minute time-series recordings. B. Immunohistochemical (IHC) staining for GCaMP-expressing cells with a GFP antibody and human cells with a human nuclear antigen (HNA) antibody. The tissue was isolated three months after xenotransplantation. C. IHC-based quantification of GCaMP labelling density between LV and AAV-labelled xenografts. The bar chart shows mean ± s.e.m. for N = 3 mice per condition. *, P = 0.0002 (two-tailed t-test). D. Representative calcium traces (shown as baseline normalized ΔF/F) recorded with 2P imaging of GCaMP8s-expressing human neurons within the xenograft; hCOs were transduced with AAV (top) or LV (bottom) before transplantation. Animals were recorded while being awake and head-fixed without presentation of external stimuli.

    Journal: bioRxiv

    Article Title: A neurorecording toolkit for longitudinal assessments of transplanted human cortical organoids in vivo

    doi: 10.64898/2025.12.20.695690

    Figure Lengend Snippet: A. Two-photon (2P) images from xenografts labelled by either AAV-hSyn-GCaMP8s (top) or LV-hSyn-GCaMP8s (bottom) in live mice one and three months after xenotransplantation. Images were generated from standard deviations of 3-minute time-series recordings. B. Immunohistochemical (IHC) staining for GCaMP-expressing cells with a GFP antibody and human cells with a human nuclear antigen (HNA) antibody. The tissue was isolated three months after xenotransplantation. C. IHC-based quantification of GCaMP labelling density between LV and AAV-labelled xenografts. The bar chart shows mean ± s.e.m. for N = 3 mice per condition. *, P = 0.0002 (two-tailed t-test). D. Representative calcium traces (shown as baseline normalized ΔF/F) recorded with 2P imaging of GCaMP8s-expressing human neurons within the xenograft; hCOs were transduced with AAV (top) or LV (bottom) before transplantation. Animals were recorded while being awake and head-fixed without presentation of external stimuli.

    Article Snippet: Two-photon imaging was performed in awake, head-fixed animals on commercial two-photon laser scanning microscope systems (Bruker Ultima, Bruker Ultima Investigator Plus) with Coherent Chameleon Ultra II or Coherent Chameleon Discovery Ti:Sapphire lasers tuned to 920-950 nm for excitation of GCaMP6s or 8s.

    Techniques: Generated, Immunohistochemical staining, Immunohistochemistry, Expressing, Isolation, Two Tailed Test, Imaging, Transduction, Transplantation Assay

    A. Experimental paradigm. For data shown in this figure, hCOs were transduced with LV-hSyn-GCaMP8s and LV-EF1-mScarlet before transplantation; animals were recorded while being awake and head-fixed without presentation of external stimuli. Schematic created with BioRender . B. Representative low-magnification 2P images (maximum intensity projections along the Z axis) of the entire optical window showing vasculature (Alexa 680-Dextran, white), GCaMP8s (green), and mScarlet (red). Abbreviations: m , medial, c , caudal. C. Two-photon images of GCaMP8s-labelled neurons in the xenograft one and three months after xenotransplantation (top) with corresponding Z-scored heat maps of calcium activity during spontaneous activity (middle) and calcium activity traces averaged across all cells (bottom). The red line on the color bar denotes the activity threshold of 10 standard deviations (Z score = 10). D. Longitudinal changes in neuronal activity over three months for each animal and imaging session (left) and averaged for pairwise comparison (right). Activity of individual cells is determined by the percentage of time the calcium trace for a given cell exceeds a threshold of 10 standard deviations (Z score >10). The scatter plot (left) shows linear mixed effects model (LMM; N=5; p<0.001, r=0.452). The scatter plot (right) shows a pairwise comparison (*, p = 0.001) paired t-test. E. Longitudinal changes in event frequency over three months for each animal and imaging session (left) and averaged for pairwise comparison (right). Events for a given cell are defined as a period where the calcium trace exceeds a threshold of 10 standard deviations (Z score >10). Data from 5 animals. The scatter plot (left) shows linear mixed effects model (LMM; N=5; p=0.0058, r=0.468). The scatter plot (right) shows a pairwise comparison (ns = not significant, p=0.088) paired t-test. F. Cell-to-cell correlation of calcium activity calculated as Pearson’s correlation coefficient between individual cells shown in panel C as measurement of synchronicity. G. Longitudinal changes in cell-to-cell synchronicity over three months for each animal and imaging (left) and averaged for pairwise comparison (right). Synchronicity is calculated as average Pearson’s correlation coefficient across all cells for each field of view. Data from 5 animals. The scatter plot (left) shows linear mixed effects model (LMM; p<0.001, r=0.759). The scatter plot (right) shows the pairwise comparison (*, p=0.026) paired t-test. H. Principal component analysis (PCA) computed from individual cells with at least one calcium event during data acquisition. Data was pooled across all imaging trials (5 animals, 144 trials, 3089 cells). Features estimated for each cell include event frequency, rhythmicity (inter-event-interval CV), activity, event length, event height, and synchronicity. PC 1 represents increases in cell activity while PC 2 represents increases in synchronicity and rhythmicity. Zones 1, 2a, and 2b define differential cell activity phenotypes within the dataset. I. PCA plot binned by month after xenotransplantation with centroid shown in red. J. Representative calcium traces for cells within Zones 1, 2a, and 2b.

    Journal: bioRxiv

    Article Title: A neurorecording toolkit for longitudinal assessments of transplanted human cortical organoids in vivo

    doi: 10.64898/2025.12.20.695690

    Figure Lengend Snippet: A. Experimental paradigm. For data shown in this figure, hCOs were transduced with LV-hSyn-GCaMP8s and LV-EF1-mScarlet before transplantation; animals were recorded while being awake and head-fixed without presentation of external stimuli. Schematic created with BioRender . B. Representative low-magnification 2P images (maximum intensity projections along the Z axis) of the entire optical window showing vasculature (Alexa 680-Dextran, white), GCaMP8s (green), and mScarlet (red). Abbreviations: m , medial, c , caudal. C. Two-photon images of GCaMP8s-labelled neurons in the xenograft one and three months after xenotransplantation (top) with corresponding Z-scored heat maps of calcium activity during spontaneous activity (middle) and calcium activity traces averaged across all cells (bottom). The red line on the color bar denotes the activity threshold of 10 standard deviations (Z score = 10). D. Longitudinal changes in neuronal activity over three months for each animal and imaging session (left) and averaged for pairwise comparison (right). Activity of individual cells is determined by the percentage of time the calcium trace for a given cell exceeds a threshold of 10 standard deviations (Z score >10). The scatter plot (left) shows linear mixed effects model (LMM; N=5; p<0.001, r=0.452). The scatter plot (right) shows a pairwise comparison (*, p = 0.001) paired t-test. E. Longitudinal changes in event frequency over three months for each animal and imaging session (left) and averaged for pairwise comparison (right). Events for a given cell are defined as a period where the calcium trace exceeds a threshold of 10 standard deviations (Z score >10). Data from 5 animals. The scatter plot (left) shows linear mixed effects model (LMM; N=5; p=0.0058, r=0.468). The scatter plot (right) shows a pairwise comparison (ns = not significant, p=0.088) paired t-test. F. Cell-to-cell correlation of calcium activity calculated as Pearson’s correlation coefficient between individual cells shown in panel C as measurement of synchronicity. G. Longitudinal changes in cell-to-cell synchronicity over three months for each animal and imaging (left) and averaged for pairwise comparison (right). Synchronicity is calculated as average Pearson’s correlation coefficient across all cells for each field of view. Data from 5 animals. The scatter plot (left) shows linear mixed effects model (LMM; p<0.001, r=0.759). The scatter plot (right) shows the pairwise comparison (*, p=0.026) paired t-test. H. Principal component analysis (PCA) computed from individual cells with at least one calcium event during data acquisition. Data was pooled across all imaging trials (5 animals, 144 trials, 3089 cells). Features estimated for each cell include event frequency, rhythmicity (inter-event-interval CV), activity, event length, event height, and synchronicity. PC 1 represents increases in cell activity while PC 2 represents increases in synchronicity and rhythmicity. Zones 1, 2a, and 2b define differential cell activity phenotypes within the dataset. I. PCA plot binned by month after xenotransplantation with centroid shown in red. J. Representative calcium traces for cells within Zones 1, 2a, and 2b.

    Article Snippet: Two-photon imaging was performed in awake, head-fixed animals on commercial two-photon laser scanning microscope systems (Bruker Ultima, Bruker Ultima Investigator Plus) with Coherent Chameleon Ultra II or Coherent Chameleon Discovery Ti:Sapphire lasers tuned to 920-950 nm for excitation of GCaMP6s or 8s.

    Techniques: Transduction, Transplantation Assay, Activity Assay, Imaging, Comparison

    A. Experimental paradigm for simultaneous two-photon (2P) calcium imaging and ECoG recordings. In this experiment, hCOs were transduced with AAV7m8 hSyn1-GCaMP6s-p2A-NLS-tdTomato in culture before xenotransplantation into retrosplenial cortex. Animals were recorded while awake and head-fixed without presentation of external stimuli. Schematic created in BioRender . B. Representative brightfield image taken at the end of the implantation surgery of the cranial exposure showing the implanted hCO xenograft and the gMEA. The gMEA is fixed to the glass window covering the exposure with optical-grade glue. Abbreviations: m , medial; c , caudal. C. Overview of the exposure acquired with 2P microscopy after labeling the blood plasma with Alexa 680-Dextran. Xenotransplant boundaries are indicated by the yellow dotted line. Graphene electrodes of the gMEA are highlighted in blue, red, and grey boxes that correspond to their location above host cortex, xenograft, and bone, respectively. The white box highlights the location of the calcium imaging field of view (FOV) for the data shown in panels D-F. D. GCaMP6s-expressing neuron recorded in the FOV shown in panel C. E. Excerpt of recorded calcium activity (shown as ΔF/F) of the neuron shown in panel D corresponding local field potential (LFP) signal recorded at the same time in channel 3 of the gMEA. F. (left) Calcium events detected in the neuron in panel D; events are aligned by calcium event onset. (right) Corresponding LFP signals in channels above the xenograft (channels 3 and 11) and above the host cortex (channels 6, 15, and 16). G. Proportion of graphene electrodes with an impedance <5 MΩ as a function of time in vivo (top) and average impedance of electrodes (with <5 MΩ) as a function of time in vivo (bottom); individual traces from six animals are shown.

    Journal: bioRxiv

    Article Title: A neurorecording toolkit for longitudinal assessments of transplanted human cortical organoids in vivo

    doi: 10.64898/2025.12.20.695690

    Figure Lengend Snippet: A. Experimental paradigm for simultaneous two-photon (2P) calcium imaging and ECoG recordings. In this experiment, hCOs were transduced with AAV7m8 hSyn1-GCaMP6s-p2A-NLS-tdTomato in culture before xenotransplantation into retrosplenial cortex. Animals were recorded while awake and head-fixed without presentation of external stimuli. Schematic created in BioRender . B. Representative brightfield image taken at the end of the implantation surgery of the cranial exposure showing the implanted hCO xenograft and the gMEA. The gMEA is fixed to the glass window covering the exposure with optical-grade glue. Abbreviations: m , medial; c , caudal. C. Overview of the exposure acquired with 2P microscopy after labeling the blood plasma with Alexa 680-Dextran. Xenotransplant boundaries are indicated by the yellow dotted line. Graphene electrodes of the gMEA are highlighted in blue, red, and grey boxes that correspond to their location above host cortex, xenograft, and bone, respectively. The white box highlights the location of the calcium imaging field of view (FOV) for the data shown in panels D-F. D. GCaMP6s-expressing neuron recorded in the FOV shown in panel C. E. Excerpt of recorded calcium activity (shown as ΔF/F) of the neuron shown in panel D corresponding local field potential (LFP) signal recorded at the same time in channel 3 of the gMEA. F. (left) Calcium events detected in the neuron in panel D; events are aligned by calcium event onset. (right) Corresponding LFP signals in channels above the xenograft (channels 3 and 11) and above the host cortex (channels 6, 15, and 16). G. Proportion of graphene electrodes with an impedance <5 MΩ as a function of time in vivo (top) and average impedance of electrodes (with <5 MΩ) as a function of time in vivo (bottom); individual traces from six animals are shown.

    Article Snippet: Two-photon imaging was performed in awake, head-fixed animals on commercial two-photon laser scanning microscope systems (Bruker Ultima, Bruker Ultima Investigator Plus) with Coherent Chameleon Ultra II or Coherent Chameleon Discovery Ti:Sapphire lasers tuned to 920-950 nm for excitation of GCaMP6s or 8s.

    Techniques: Imaging, Transduction, Microscopy, Labeling, Clinical Proteomics, Expressing, Activity Assay, In Vivo